MDM2 (Phospho-Tyr394) Rabbit pAb
Sizes: 50μL, 100μL
Catalogue Numbers: ES8584-50, ES8584-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],
Alternate Name: E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (Hdm2) (Oncoprotein Mdm2) (p53-binding protein Mdm2)
Source: Rabbit
Applications: WB; ELISA
Dilution: WB 1:500-2000, ELISA 1:10000-20000
Reactivity: Human; Mouse
Immunogen: Synthesized phospho-peptide around the phosphorylation site of human MDM2 (Phospho-Tyr394)
Storage and Stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed Band (KD): 42kD
Human Gene ID: 4193
Human SWISS Prot NO: Q00987
Subcellular Location: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Nucleus. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF (P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
Research Use Only