Anti-Dupilumab Neutralizing Antibody ELISA kit
Size: 96T
Catalogue Number: AB732518-96
Citations, Manuals and MSDS Available upon request.
Description: This assay employs the quantitative competitive enzyme immunoassay technique. Dupilumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD124 antigen and then pipetted into the wells. Anti-Dupilumab Neutralizing Antibody in the sample competitively binds to Dupilumab with the biotin-labeled Human CD124 antigen. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Dupilumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Stability and Storage: When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
Detection Method: Colorimetric
Sample Type: Plasma, Serum
Assay Type: Quantitative
Sensitivity: 82.6 ng/mL
Range: 156.25 - 10,000 ng/mL
Recovery: 80-120%
Shipping: 2-8 ℃
For Research Use Only.