Anti-SARS-CoV-2 Nucleocapsid (NP) Human IgM ELISA Kit
Size: 96T
Catalogue Number: AK575038-96
Citations, Manuals and MSDS Available upon request.
Description: This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 Nucleocapsid (NP) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Nucleocapsid (NP) Human IgM present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgM antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 Nucleocapsid (NP) Human IgM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Stability and Storage: When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
Detection Method: Colorimetric
Sample Type: Plasma, Serum
Assay Type: Quantitative
Sensitivity: 2.66 ng/mL
Range: 15.63 - 1,000 ng/mL
Recovery: 80-120%
Shipping: 2-8 ℃
For Research Use Only.