Trastuzumab ELISA Kit
Size: 96T
Catalogue Number: DY286038-96
Citations, Manuals and MSDS Available upon request.
Description: This assay employs the quantitative competitive enzyme immunoassay technique. A recombinant human ErbB2 antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells.Trastuzumab in the sample competitively binds to the pre-coated protein with biotin-labeled Trastuzumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Trastuzumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Stability and Storage: When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
Detection Method: Colorimetric
Sample Type: Plasma, Serum
Assay Type: Quantitative
Sensitivity: 70.12 ng/mL
Range: 78.13 - 5,000 ng/mL
Recovery: 80-120%
Shipping: 2-8 ℃
For Research Use Only.