14-3-3 α/β Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90005-50, BS90005-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P31946(Human) Q9CQV8(Mouse) P35213(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC, IP, FC
All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: 14-3-3 α/β polyclonal antibody detects endogenous levels of 14-3-3 α/β protein.
Bioworld Molecular Weight: 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: HepG2, ICC staining 14-3-3 alpha+beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: KCIP-1, YWHAB, 14 3 3 alpha, 14 3 3 protein beta/alpha, 14-3-3 protein beta/alpha, 1433B, Brain protein 14 3 3 beta isoform, GW128, HS 1, KCIP-1, KCIP1, N-terminally processed, Protein 1054, Protein kinase C inhibitor protein 1, YWHAA, YWHAB,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified