14-3-3 ζ (phospho-S58) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS4001-50, BS4001-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P63104
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: p-14-3-3 ζ (S58) polyclonal antibody detects endogenous levels of 14-3-3 protein zeta/delta only when phosphorylated at Ser58.
Bioworld Molecular Weight: ~ 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of 14-3-3 ζ (phospho-S58) polyclonal antibody at 1:500 dilution
Lane 1: The Brain tissue lysate of Mouse (40ug)
Lane 2: The Brain tissue lysate of Rat (40ug)
Lane 3: HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate (40ug)
Lane 4: HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate (40ug)
Lane 5: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (phospho-S58) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (phospho-S58) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (phospho-S58) pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ; 14-3-3 protein zeta; 14-3-3 protein delta; 14-3-3 ζ; 14-3-3 δ; 1433Z; KCIP1; KCIP 1
Immunogen: Synthetic phosphopeptide derived from human Histone 14-3-3 ζ around the phosphorylation site of Serine 58.
Conjugate: Unconjugated
Modification: Phosphorylation