14-3-3 η (K81) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS2384-50, BS2384-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q04917
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200
Background: 14-3-3 eta (YWHAH) belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine or phosphothreonine-containing proteins. YWHAH is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. It binds to a large number of partners and binding generally results in the modulation of the activity of the binding partner. The YWHAH gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: 14-3-3 η (K81) polyclonal antibody detects endogenous levels of 14-3-3 η protein.
Bioworld Molecular Weight: ~ 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of 14-3-3 η (K81) pAb at 1:1000 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: The Brain tissue lysate of Mouse (20ug)
Lane 4: The Brain tissue lysate of Rat (20ug), Immunohistochemistry (IHC) analyzes of 14-3-3 η (K81) pAb in paraffin-embedded human breast carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 η (K81) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 η (K81) pAb in paraffin-embedded human rectum carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: 14-3-3 protein eta; Protein AS1; YWHAH; YWHA1; 1433F
Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human 14-3-3 η.
Conjugate: Unconjugated
Modification: Unmodified