14-3-3 sigma Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90003-50, BS90003-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P31947(Human) EntrezGene:313017(Rat)
Host: Rabbit
Reactivity: Human, Rat
Applications: WB, IHC
All Applications: WB:1:1,000
IHC:1:50-1:200
Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: 14-3-3 sigma polyclonal antibody detects endogenous levels of 14-3-3 sigma protein.
Bioworld Molecular Weight: 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin., Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.
Alternative Name: SFN, 14 3 3 protein sigma, 14-3-3 protein sigma, 1433S, Epithelial cell marker protein 1, Er, HME 1, HME1, MGC143283, Mkrn3, Mme1, OTTHUMP00000004242, RP23 137L22.11, SFN, SFN protein, Stratifin, YWHAS,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified