4E BP1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BZ16283-50, BZ16283-100
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Swiss-Prot: Q13541
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IP,
All Applications: WB: 1/500-1/1000 IP: 1/20
Background: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 13 kDa; Observed MW: 15-20 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of 4EBP1 in SH-SY5Y, C2C12 and 3T3 lysates using 4EBP1 antibody., Immunoprecipitation analysis of 4E BP1 in Hela lysates using 4EBP1 antibody., Western blot analysis of 4E BP1 in Jurkat, Hela and C6 lysates using 4EBP1 antibody., Western blot analysis of 4E BP1 in C2C12, A549 and 3T3 lysates using 4E BP1 antibody
Alternative Name: EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Immunogen: Synthetic peptide.
Conjugate: Unconjugated
Modification: Unmodified