4E BP1 Polyclonal Antibody
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Sizes: 50µl, 100µl
Catalogue Numbers: BZ16996-50, BZ16996-100
Swiss-Prot: Q13541
Host: Rabbit
4E BP1 Polyclonal Antibody-BZ16996
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human
Applications: WB, IHC-P, FC, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20 FC: 1/50-1/100
Background: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.
Purification and Purity: Affinity Purified
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 13 kDa; Observed MW: 15-20 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of 4EBP1 in K562, 293, HelaLysates using 4E BP1 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using eIF4EBP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Immunogen: A synthetic peptide of human eIF4EBP1
Modification: Unmodified
Conjugate: Unconjugated