A2M Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90012-50, BS90012-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01023(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, IHC
All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200
Background: α-2-Macroglobulin (α-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, α-2M was characterized as a pan-proteinase inhibitor that was able to "bait" proteinases into cleaving specific peptide sequences on α-2M. This interaction induces a conformational change in α-2M, thus enabling it to "trap" the proteinase and further inhibit its activity. Subsequently, α-2M has been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor β (TGFβ) in serum is primarily bound to α-2M, which renders TGFβ inactive. α-2M also binds to IL-6 and, thereby, increases the concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved in mediating the inflammatory cascade. Mutations and deletions in the gene encoding α-2M are associated with an increased incidence of Alzheimer's Disease (AD), which is consistent with the role of α-2M in mediating the clearance and degradation of A β, the major component of β-Amyloid deposits accumulated during AD.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: A2M polyclonal antibody detects endogenous levels of A2M protein.
Bioworld Molecular Weight: 163 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of A2M on human placenta cells lysates using anti-A2M antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- A2M antibody. Counter stained with hematoxylin.
Alternative Name: Alpha-2-M, A2M, A2m, A2MG, Alpha 2 M, Alpha 2M, Alpha-2-M, Alpha-2-macroglobulin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, CPAMD5, DKFZp779B086, FWP007, S863 7,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified