ABCB4 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS79273-50, BS79273-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P21439
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 144kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of mouse brain, using ABCB4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Alternative Name: ABCB4; ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3; PFIC-3; PGY3
Immunogen: Recombinant fusion protein of human ABCB4(NP_061337.1).
Conjugate: Unconjugated
Modification: Unmodified