ABCG8 Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50 µl, 100 µl
Catalogue Numbers: BS6865-50, BS6865-100
Swiss-Prot: Q9H221
Host: Rabbit
ABCG8 Polyclonal Antibody-BS6865
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 76KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of LO2 cells, using ABCG8 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 18s., Immunofluorescence analysis of HeLa cells using ABCG8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: ABCG8; GBD4; STSL
Immunogen: Recombinant fusion protein of human ABCG8(NP_071882.1).
Modification: Unmodified
Conjugate: Unconjugated