ACER1 Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS60096-50, BS60096-100
Sizes: 50µl, 100µl
Swiss-Prot: Q8TDN7
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: ASAH3 (N-acylsphingosine amidohydrolase (alkaline ceramidase) 3), also designated ACER1, is a 264 amino acid multi-pass membrane protein that localizes to the membrane of the endoplasmic reticulum. Expressed predominately in epidermal tissue, ASAH3 catalyzes the hydrolysis of the sphingolipid ceramide into sphingosine and a free fatty acid, thereby playing an important role in the regulation of bioactive ceramide and sphingosine levels within the cell. ASAH3 functions at an optimal pH of 8.0 and has high specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine, but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: ACER1 polyclonal antibody detects endogenous levels of ACER1 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ACER1 polyclonal antibody at 1:500 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of ACER1 pAb bin paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 50 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Alkaline ceramidase 1; AlkCDase 1; Alkaline CDase 1; EC=3.5.1.23; Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; ACER1; ASAH3
Immunogen: A synthetic peptide corresponding to residues in Human ACER1
Conjugate: Unconjugated
Modification: Unmodified