Acetyl-Histone H2A-K5 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS74027-50, BS74027-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P0C0S8
Host: Rabbit
Reactivity: Human, Mouse, Rat, Other (Wide Range)
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Acetylated Antibodies
Bioworld Molecular Weight: 14kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody at dilution of 1:100.C6 cells were treated by TSA at 37℃ for 18 hours. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody at dilution of 1:100.HeLa cells were treated by TSA at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
Alternative Name: HIST1H2AI; H2A/c; H2AFC
Immunogen: A synthetic acetylated peptide around K5 of human Histone H2A(NP_003508.1).
Conjugate: Unconjugated
Modification: Acetylated