Acetyl-Histone H2B (Lys5) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BZ17068-50, BZ17068-100
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Swiss-Prot: P33778
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-F, IHC-P, ICC/IF, IP, , ChIP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20
Background: Belongs to the histone H2B family. Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 14 kDa; Observed MW: 14 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Acetyl-Histone H2B in various cell lines lysates using Acetyl-Histone H2B-K5 antibody., Immunofluorescence analysis of Acetyl-Histone H2B in C6 using Acetyl-Histone H2B-K5 antibody.C6 cells were treated by TSA,and DAPI., Immunofluorescence analysis of Acetyl-Histone H2B in HeLa cells using Acetyl-Histone H2B-K5 antibody.HeLa cells were treated by TSA,and DAPI., Immunofluorescence analysis of Acetyl-Histone H2B in NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody, and DAPI.NIH/3T3 cells were treated by TSA.
Alternative Name: H2BK5ac; H2B 1A; H2B; H2B histone family; H2B2f; H2Ba; H2Bf; HIST2H2BF; histone H2B; histone H2B type 1; Histone H2B type 2-F
Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B
Conjugate: Unconjugated
Modification: Acetylated