Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS79335-50, BS79335-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P68431
Host: Rabbit
Reactivity: Human, Mouse, Rat, Other (Wide Range)
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 antibody at 1:610 dilution. HeLa cells,NIH/3T3 cells and C6 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Bioworld Molecular Weight: 17kDa
Specificity: Acetylated
Alternative Name: H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A
Immunogen: A synthetic acetylated peptide around K4 & K27 of human Acetyl-Histone H3-K4/K9/K14/K18/K23/K27(NP_003520.1).
Conjugate: Unconjugated
Modification: Acetylated