Acetyl-Histone H3-K9 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS74033-50, BS74033-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q16695
Host: Rabbit
Reactivity: Human, Mouse, Rat, Other (Wide Range)
Applications: WB, IHC, IF/ICC, IP, , ChIP, , ChIP, -seq
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200 | ChIP,1:20 - 1:100 | ChIP-seq,1:20 - 1:50
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Acetylated Antibodies
Bioworld Molecular Weight: 17KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody at 1:1000 dilution.HeLa acid extract and NIH/3T3 acid extract and C6 acid extract were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Alternative Name: H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Immunogen: A synthetic acetylated peptide around K9 of human Histone H3(NP_003520.1).
Conjugate: Unconjugated
Modification: Acetylated