Acetyl-Histone H4-K5 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS79333-50, BS79333-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P62805
Host: Rabbit
Reactivity: Human, Mouse, Rat, Other (Wide Range)
Applications: WB, IHC, ChIP,
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | ChIP,1:50 - 1:200
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Acetylated
Bioworld Molecular Weight: 11kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody at 1:1000 dilution.HeLa cells,NIH/3T3 cells and C6 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Alternative Name: H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; HIST2H4; HIST2H4A
Immunogen: A synthetic acetylated peptide around K5 of human Histone H4(NP_001029249.1).
Conjugate: Unconjugated
Modification: Acetylated