AChE (K585) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS3694-50, BS3694-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P22303
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IHC
All Applications: IHC:1:50~1:200
Background: Acetylcholinesterase (AChE) hydrolyzes acetylcholine at synaptic junctions.Alternative mRNA splicing gives rise to three forms of AChE. The T form, also known as the asymmetric form, is soluble and is present in synapses. The H form is also known as the globular form and is present on the outer surfaces of cell membranes. The R form is not known to be a functional species. AChE globular form subunits are GPI-anchored to cell membranes and asymmetric subunits are anchored to basal lamina components by a collagen tail. The catalytic subunits of AChE are oligomers composed of disulfide-linked homodimers. The loss of AChE from cholinergic and noncholinergic neurons in the brain is seen in patients with Alzheimer’s disease. However, AChE activity is increased around amyloid plaques, which may be due to a disturbance in calcium homeostasis involving the opening of L-type voltage-dependent calcium channels.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AChE (K585) polyclonal antibody detects endogenous levels of AChE protein.
Bioworld Molecular Weight: ~ 70 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry (IHC) analyzes of AChE (K585) pAb bin paraffin-embedded human lung carcinoma tissue at 1:50, showing membrane and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of AChE (K585) pAb bin paraffin-embedded human rectum carcinoma tissue at 1:50, showing membrane and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Acetylcholinesterase; AChE; ACHE; ACES
Immunogen: Synthetic peptide, corresponding to amino acids 560-600 of Human AChE.
Conjugate: Unconjugated
Modification: Unmodified