ACHE Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS78387-50, BS78387-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P22303
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 75kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ACHE antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of NIH/3T3 cells using ACHE Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using ACHE Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of C6 cells using ACHE Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: ACHE; ACEE; ARACHE; N-ACHE; YT
Immunogen: Recombinant fusion protein of human ACHE(NP_056646.1).
Conjugate: Unconjugated
Modification: Unmodified