AChRα3 (L139) Polyclonal Antibody
Catalogue Numbers: BS2851-50, BS2851-100
Sizes: 50µl, 100µl
Alternative Name: Neuronal acetylcholine receptor subunit alpha-3; NACHRA3; CHRNA3
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P32297
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The nicotinic acetylcholine receptor (nAChR) is a ligand gated ion channel that mediates neurotransmission at the neuromuscular junction, autonomic ganglia and at some sites in the central nervous system. Distinct nAChR subtypes exist that can be stimulated by the neurotransmitter acetylcholine, the natural product nicotine, or by synthetic compounds. After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 3 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AChRα3 (L139) polyclonal antibody detects endogenous levels of Neuronal acetylcholine receptor subunit α3 protein.
Bioworld Molecular Weight: ~ 60 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AChRα3 (L139) pAb at 1:500 dilution
Lane 1: The Brain tissue lysate of Mouse(40ug)
Lane 2: C6 whole cell lysate(40ug)
Lane 3: A549 whole cell lysate(40ug)
Lane 4: Hela whole cell lysate(40ug)
Immunogen: Synthetic peptide, corresponding to amino acids 120-180 of Human AChRα3.
Conjugate: Unconjugated
Modification: Unmodified