Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) CLIA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-CL-H0832-24, E-CL-H0832-48, E-CL-H0832-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: HIF-1α
UNIProt ID: Q16665
Target Synonyms: HIF1A; HIF-1A; MOP1; PASD8; bHLHe78; basic helix-loop-helix transcription factor;
Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism
Target Species: Human
Application: CLIA
Usage: This ELISA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in Tissue homogenates,cell lysates and other biological fluids.
Tested Sample Types: Tissue homogenates,cell lysates and other biological fluids
Type: Sandwich
Assay Time: 3.5h
Detection Type: Chemiluminescence
Sensitivity: 18.75 pg/mL
Detection Range: 31.25-2000 pg/mL
Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HIF-1α. You can calculate the concentration of Human HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.
Cross Reactivity: This kit recognizes Human HIF-1α in samples. No significant cross-reactivity or interference between Human HIF-1α and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃/-20℃, 6 months
Shipping: ice bag