Rat PFK-A (6-phosphofructokinase Type A) CLIA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-CL-R0714-24, E-CL-R0714-48, E-CL-R0714-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: PFK-A
UNIProt ID: P47858
Target Synonyms: PFK-M; ATP-PFK; ATP-dependent 6-phosphofructokinase; muscle type; Phosphofructo-1-kinase isozyme A; Phosphohexokinase;
Research Areas: Metabolism, Signal Transduction , Cancer
Target Species: Rat
Application: CLIA
Usage: This ELISA kit applies to the in vitro quantitative determination of Rat PFK-A concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, Plasma and other biological fluids
Type: Sandwich
Assay Time: 3.5h
Detection Type: Chemiluminescence
Sensitivity: 0.19 ng/mL
Detection Range: 0.31-20 ng/mL
Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PFK-A. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PFK-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PFK-A, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PFK-A. You can calculate the concentration of Rat PFK-A in the samples by comparing the RLU value of the samples to the standard curve.
Cross Reactivity: This kit recognizes Rat PFK-A in samples. No significant cross-reactivity or interference between Rat PFK-A and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃/-20℃, 6 months
Shipping: ice bag