Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
Sizes: 24T, 48T, 96T
Catalogue Numbers: WEC918Ra-24, WEC918Ra-48, WEC918Ra-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Wide range kit
Target: Fibroblast Growth Factor 21
Target Species: Rat
Uniprot: Q8VI80
Gene ID: 170580
Featured Series Function: Detects protein (wildest detection range for analytes from different samples type)
Specificity: Reactive with Rat FGF21 / Fibroblast Growth Factor 21
Method: Colormetric
Detection principle: Competitive Inhibition
Detection
range: 12.35-1,000pg/mL
Sensitivity: 4.77pg/mL
Assay Time: 3h
Sample Size: 50uL
Recommended/Predicted
Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Fibroblast Growth Factor 21 (FGF21).
No significant cross-reactivity or interference between Wide-range Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Fibroblast Growth Factor 21 (FGF21) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range Fibroblast Growth Factor 21 (FGF21) and unlabeled Wide-range Fibroblast Growth Factor 21 (FGF21) (Standards or samples) with the pre-coated antibody specific to Wide-range Fibroblast Growth Factor 21 (FGF21). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range Fibroblast Growth Factor 21 (FGF21) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range Fibroblast Growth Factor 21 (FGF21) in the sample.
Research Area: Cytokine;
Research Use Only