Recombinant BMP-3B, Human
Catalogue Numbers: -10, -50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 25.1 kDa, observed by non-reducing SDS-PAGE.
Purity: > 95% as analyzed by SDS-PAGE and HPLC.
Biological Activity:
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against 4mM HCl.
AA Sequence: MQWDEPRVC SRRYLKVDFA DIGWNEWIIS PKSFDAYYCA GACEFPMPKI VRPSNHATIQ SIVRAVGIIP GIPEPCCVPD KMNSLGVLFL DENRNVVLKV
YPNMSVDTCA CR
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in 4mM HCl at 100 μg/mL.
Storage: Lyophilized recombinant human Bone Morphogenetic Protein-3B (rhBMP-3B) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhBMP-3B remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Bone Morphogenetic Protein-3B (BMP-3B) , also known as Growth/Differentiation Factor 10 (GDF-10), is a cytokine belonging to the Transforming Growth Factor β (TGF-β) superfamily. BMP-3B contains the cystine knot motif shared by other TGF-β family members. BMP-3B was originally identified by PCR based on the BMP-3 sequence, and shares 83% identity with BMP-3. BMP-3B and BMP-2 act as mutual antagonists, as they compete for the availability of signaling protein Smad4. In vivo, BMP-3B is highly expressed in brain, lungs, and bone tissues. The functions of BMP-3B include acting as a dorsaling factor in head development, inhibition of adipogenesis in adipocytes, and induction of bone formation. BMP-3B is down-regulated in lung cancer patient samples, indicating its potential antitumor activity. Recombinant human Bone Morphogenetic Protein-3B (rhBMP-3B) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 111 amino acids each. rhBMP-3B has a molecular mass of 25.1 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.