Recombinant IGF-I, Rat
Catalogue Numbers: BK0086-10, BK0086-50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 7.8 kDa, observed by reducing SDS-PAGE.
Purity: > 95% as analyzed by SDS-PAGE and HPLC.
Biological Activity: ED50 < 10 ng/mL, measured by a cell proliferation assay using FDCP-1 cells, corresponding to a specific activity of > 1×10ˆ5 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: MGPETLCGA ELVDALQFVC GPRGFYFNKP TGYGSSIRRA PQTGIVDECC FRSCDLRRLE MYCAPLKPTK SA
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/mL.
Storage: Lyophilized recombinant rat Insulin-like Growth Factor I (rrIGF-I) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrIGF-I remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Insulin-like Growth Factor I (IGF-I) is a single chain 7 kDa growth-promoting polypeptide originally identified as somatomedin-c. It belongs to the IGF family of peptides, which also includes IGF-II and insulin. The gene expression of IGF-I is mainly regulated by Growth Hormone, and IGF-I executes its functions via signaling through transmembrane tyrosine receptors(IGF Receptors). Most circulating IFG-I is associated with the IGF Binding Protein 3 (IGFBP-3), and the IGFBPs may inhibit the actions of IGFs by competing against the IGF Receptors. IGF-I is active in post-natal and adult animals, and is crucial for somatic growth, as IGF-I null mice show marked retardation in utero. IGF-I is involved in the carcinogenesis, and related to the prostate cancer as well. Recombinant rat Insulin-like Growth Factor I (rrIGF-I) produced in E.coli is a single non-glycosylated polypeptide chain containing 71 amino acids. A fully biologically active molecule, rrIGF-I has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.