Recombinant IL-6, Human
Catalogue Numbers: BK0109-10, BK0109-50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 20.9 kDa, observed by reducing SDS-PAGE.
Purity: > 95% by SDS-PAGE analysis.
Biological Activity: ED50 <0.1ng/ml, measured by the dose-dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells, corresponding to a specific activity of > 1 x 10ˆ7 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against 1xPBS.
AA Sequence: MPVPPGEDS KDVAAPHRQP LTSSERIDKQ IRYILDGISA LRKETCNKSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEETCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PDPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSSL RALRQM
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/ml.
Storage: Lyophilized recombinant human Interleukin-6 (rhIL-6) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-6 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Interleukin-6 (IL-6, also known as IFN-β2) is a pleiotropic cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 is secreted by T cells and macrophages to stimulate immune response. IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells.Recombinant human Interleukin-6 (rhIL-6) produced in E.coli is a single non-glycosylated polypeptide chain containing 185 amino acids. A fully biologically active molecule, rhIL-6 has a molecular mass of 20.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.