Recombinant LR3-IGF-I(ReceptorGrade), Human
Catalogue Numbers: BK0123-100, BK0123-10
Sizes: 100μg, 10μg
Source: Escherichia coli
Molecular Weight: 9.1±0.9 KDa, observed by reducing SDS-PAGE.
Purity: > 95% by SDS-PAGE.
Biological Activity: ED50 ＜10 ng/ml, measured by the dose-dependantproliferation of CHO cells, corresponding to a specific activity of >1.0× 10ˆ5 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after dissolved in 48%acetonitrile and 0.1% TFA
AA Sequence: MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK
PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA
KSA
Endotoxin: ＜1 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in 10mM HCl at 1mg/ml. Use a 0.22 μm membrane for filter Sterilization.
Storage: Lyophilized recombinant human Long R3Insulin-like Growth Factor-1 (rhLR3IGF-1) remains stable up to 6 months at -80°C from date of receipt. Stock solution of peptide can be stored at least 3 months at -20°C. Avoid repeated freeze-thaw cycles.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: IGF-1 is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-1 is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-2, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-1 (rhLR3IGF-1) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3)and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs. RhLR3IGF-1 is properly folded under oxidizing conditions and then purified by proprietary chromatographic techniques at GenScript.