Recombinant Mouse Dendritic Cell Inflammatory Protein 1/CXCL3 (rMuDCIP-1/CXCL3)
Catalogue Numbers: PR2001-2, PR2001-10
Sizes: 2µg, 10µg
Source: Escherichia coli
Molecular Weight: 7.7 kDa, a single, non-glycosylated polypeptide chain containing 69 amino acids.
Purity: >97% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. Determined by its ability to chemoattract 293 transfected CXCR2 cells using a concentration range of 10-100 ng/ml, corresponding to a Specific Activity of ＞1 x 104 IU/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
AA Sequence: SELRCQCLNT LPRVDFETIQ SLt VTPPGPH CTQTEVIATL KDGQEVCLNP
QGPRLQIIIK KILKSGKSS
Endotoxin: Less than 1EU/mg of rMuDCIP-1/CXCL3 as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China
Description: CXCL3 is also known as MIP2β (macrophage inflammatory protein 2 beta), or DCIP1 (dendritic cell inflammatory protein1) in mouse, CINC2 (cytokineinduced neutrophil attractant 2) in rat, and GROγ (growth regulated oncogene gamma) in humans. It is an 8 kDa proinflammatory member of the CXC subfamily of heparinbinding chemokines, also called alpha chemokines. Mature mouse CXCL3 shares 88% and 57% amino acid (aa) sequence identity with rat and human CXCL3, respectively.