Recombinant NANOG-TAT, Human
Catalogue Numbers: BK0142-20, BK0142-100
Sizes: 20μg, 100μg
Source: Escherichia coli
Molecular Weight: 36.2 kDa, analyzed by reducing SDS-PAGE.
Purity: > 95% by SDS-PAGE and HPLC analyses.
Biological Activity:
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Sterile Filtered solution contains 10mM PB, 300mM NaCl, pH7.4.
AA Sequence: MSVDPACPQ SLPCFEASDC KESSPMPVIC GPEENYPSLQ MSSAEMPHTE t VSPLPSSMD LLIQDSPDSS TSPKGKQPTS AENSVAKKED KVPVKKQKTR t VFSSTQLCV LNDRFQRQKY LSLQQMQELS NILNLSYKQV KTWFQNQRMK SKRWQKNNWP KNSNGVTQKA SAPTYPSLYS SYHQGCLVNP TGNLPMWSNQ TWNNSTWSNQ TQNIQSWSNH SWNTQTWCTQ SWNNQAWNSP FYNCGEESLQ SCMQFQPNSP ASDLEAALEA AGEGLNVIQQ TTRYFSTPQT MDLFLNYSMN MQPEDVGGYG RKKRRQRRR
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.
Storage: Recombinant human NANOG-TAT (rhNANOG-TAT) remains stable up to 1-2 weeks at 4°C from date of receipt. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freezing and thawing cycles.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E.coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.