Recombinant NAP-2/CXCL7, Rat
Catalogue Numbers: BK0143-5, BK0143-25
Sizes: 5μg, 25μg
Source: Escherichia coli
Molecular Weight: 6.9 kDa, observed by reducing SDS-PAGE.
Purity: > 95% as analyzed by SDS-PAGE.
Biological Activity: The EC50 value of Rat NAP-2/CXCL7on Caˆ2+ mobilization assay in CHO-K1/Gα15/rCXCR2 cells (human Gα15 and Rat CXCR2 stably expressed in CHO-K1 cells) is less than 200 ng/ml.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: IELRCRCTNT LSGIPLNSIS RVNVFRPGAH CDNVEVIATL KNGKEVCLDP
TAPMIKKIVK KI
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.
Storage: Lyophilized recombinant Rat NAP-2/CXCL7 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Rat NAP-2/CXCL7 should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Rat NAP-2/CXCL7 is a small cytokine belonging to the CXC chemokine family. It is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein (PPBP). Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR-2 and recruit and active ateneutrophils through chemotaxis. Although CTAP-III, β-TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. NAP-2 stimulates various processes including mitogenesis, synthesis of the extracellular matrix, glucose metabolism and synthesis of plasminogen activator. Recently, it has been shown that the additional amino-terminal residues of CTAP-III mask the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.Recombinant Rat NAP-2 /CXCL7 produced in E.coli is a single non-glycosylated polypeptide chain containing 62 amino acids. A fully biologically active molecule, rrNAP-2/CXCL7 has a molecular mass of 6.9 kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.