Recombinant PDGF-BB, Rat
Catalogue Numbers: BK0153-10, BK0153-50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 24.6 kDa, observed by non-reducing SDS-PAGE.
Purity: > 95% by SDS-PAGE analysis.
Biological Activity: ED50 < 2 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5 × 10ˆ5 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against 20 mM acetic acid.
AA Sequence: MSLGSLAAA EPAVIAECKT RTEVFQISRN LIDRTNANFL VWPPCVEVQR CSGCCNNRNV QCRASQVQMR PVQVRKIEIV RKKPVFKKAT VTLEDHLACK CEt VVTPRPV T
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in 20 mM acetic acid at 100 μg/mL.
Storage: Lyophilized recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrPDGF-BB remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rrPDGF-BB has a molecular mass of 24.6 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.