Human aFP (Alpha-Fetoprotein) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1301MS-48, ELK1301MS-96, ELK1301MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor
Assay Type: Sandwich
Sensitivity: 5.22 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Cytokine; Apoptosis; Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF15, and the Human GDF15 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human GDF15. After TMB substrate solution is added, only those wells that contain Human GDF15 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q99988
Research Use Only