Human IL1b (Interleukin 1 Beta) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1270MS-48, ELK1270MS-96, ELK1270MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
Assay Type: Sandwich
Sensitivity: 31 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.2-5000 pg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Cytokine; Apoptosis; Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P15692
Research Use Only