Acid Phosphatase (ACP) Activity Assay Kit (PNPP Method)
Sizes: 96T, 500 Assays
Catalogue Numbers: E-BC-K010-M-96, E-BC-K010-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: ACP
Research Areas: Liver and Renal Function
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, tissue
Assay Time: 30 min
Detection Type: Colorimetric method
Sensitivity: 0.2 U/L
Detection Range: 0.2–50 U/L
Test Principle: Disodium p-nitrobenzene phosphate (p-NPP) , a widely used phosphatase chromogenic substrate, can form p- nitrophenol under the action of acid phosphatase. Under alkaline conditions, p-nitrophenol is yellow and has a maximum absorption peak at 405 nm. The darker of the yellow product is, the higher of the ACP activity is. Therefore, the activity of ACP can be calculated by measuring the OD value at 405 nm.
Inter CV: 4.5
Intra CV: 2.8
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag