AF/LE Purified Anti-Mouse CD45R/B220 Antibody [RA3.3A 1/6.1] - E-AB-F11120

AF/LE Purified Anti-Mouse CD45R/B220 Antibody [RA3.3A 1/6.1]

Sizes: 500μg, 1mg

Catalogue Numbers: E-AB-F11120-500, E-AB-F11120-1

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD45R/B220

Target Synonym: B220,

Conjugation: None (Purified antibody-Azide Free/Low endotoxin)

Host: Rat

Species Reactivity: Mouse

Application: FCM,Cell Sep-Neg,Depletion

Usage: Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10^6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype: Rat IgM, κ

Clone No.: RA3.3A 1/6.1

Background: CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cell specificity.

Concentration: 0.5 mg/mL

Control Catalogue Number: E-AB-F097730

Buffer: 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.

Gene ID: 192645788

Storage: This product can be stored at 2-8°C for 12 months. Do not freeze.

Shipping: Ice bag

Research Use Only