Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-F038-48, E-BC-F038-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: ALT
Research Areas: Liver and Renal Function
Result Type: Enzyme Activity
Detection Instrument: Fluorescence Microplate Reader
Tested Sample Types: Serum, plasma, animal tissue
Assay Time: 75 min
Detection Type: Fluorescence method
Sensitivity: 0.01 U/L
Detection Range: 0.01-0.83 U/L
Test Principle: ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Inter CV: 9.8
Intra CV: 2.3
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag