APAF1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-12215-60, E-AB-12215-120, E-AB-12215-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: APAF1
Target Synonym: APAF 1; Apaf-1; APAF; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413
Research Areas: Cancer, Cell Biology, Metabolism
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: O14727
Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Concentration: 0.7 mg/mL
Immunogen: Synthetic peptide of human APAF1
Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:50-1:200
Calculated MW: 142kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only