APAF1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-65705-60, E-AB-65705-120, E-AB-65705-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: APAF1
Target Synonym: APAF1; APAF-1; CED4
Research Areas: Cancer, Cell Biology, Metabolism
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: O14727
Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human APAF1 (NP_863651.1).
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000
Calculated MW: 37kDa/133kDa/135kDa/137kDa/140kDa/141kDa
Observed MW: 142kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only