KI67 Monoclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-22027-60, E-AB-22027-120, E-AB-22027-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: Ki 67
Target Synonym: Antigen identified by monoclonal Ki 67; Antigen identified by monoclonal Ki-67; Antigen KI-67; Antigen KI67; Antigen Ki67; KI67; KIA; Marker of proliferation Ki-67; MIB 1; MIB; MKI67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen; Protein phosphatase 1 regulatory subunit 105; RP11-380J17.2
Research Areas: Cancer, Cell Biology, Neuroscience, Tags and Cell Markers
Conjugation: Unconjugated
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Application: IHC-p, IF
Isotype: IgG
Clonality: Monoclonal
Clone No.: Clone:8K5
UNIProt ID: P46013
Background: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Concentration: 1 mg/mL
Immunogen: Synthetic Peptide
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method: Protein A purification
Dilution: IHC 1:100-1:300, IF 1:50-200
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only