(KO Validated) AKT1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-93290-60, E-AB-93290-120, E-AB-93290-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: AKT1
Target Synonym: AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT serine/threonine kinase 1
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P31749
Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Concentration: 1mg/mL
Immunogen: A synthetic peptide of human AKT1
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200
Calculated MW: 48kDa/55kDa
Observed MW: 60KDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only