MANEA Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-90243-60, E-AB-90243-120, E-AB-90243-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: MANEA
Target Synonym: MANEA; ENDO; hEndo
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q5SRI9
Background: N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1, 2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008]
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human MANEA
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000
Calculated MW: 53kDa
Observed MW: 54kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only