MAP3K5 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-93036-60, E-AB-93036-120, E-AB-93036-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: MAP3K5
Target Synonym: MAP3K5; ASK1; MAPKKK5; MEKK5
Research Areas: Cancer, Cell Biology, Cardiovascular
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q99683
Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human MAP3K5
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000
Calculated MW: 69kDa/154kDa
Observed MW: 155kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only