Mouse IgG2a (Immunoglobulin G2a) CLIA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-CL-M0429-24, E-CL-M0429-48, E-CL-M0429-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: IgG2a
UNIProt ID: P01863
Target Synonyms: IgG2a
Research Areas: Immunology
Target Species: Mouse
Application: CLIA
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse IgG2a concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, Plasma and other biological fluids
Type: Sandwich
Assay Time: 3.5h
Detection Type: Chemiluminescence
Sensitivity: 0.94 ng/mL
Detection Range: 1.56-100 ng/mL
Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2a. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG2a, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IgG2a. You can calculate the concentration of Mouse IgG2a in the samples by comparing the RLU value of the samples to the standard curve.
Cross Reactivity: This kit recognizes Mouse IgG2a in samples. No significant cross-reactivity or interference between Mouse IgG2a and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃/-20℃, 6 months
Shipping: ice bag