Mouse MFGE8 (Milk Fat Globule EGF Factor 8) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-EL-M3089-24, E-EL-M3089-48, E-EL-M3089-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: MFGE8
UNIProt ID: P21956
Target Synonyms: HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8
Research Areas: Cancer, Cardiovascular, Developmental biology, Microbiology
Target Species: Mouse
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse MFGE8 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: Serum, Plasma and other biological fluids
Type: Sandwich
Assay Time: 3.5h
Detection Type: Colormetric
Sensitivity: 5.46 pg/mL
Detection Range: 31.25-2000 pg/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MFGE8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MFGE8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MFGE8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MFGE8. You can calculate the concentration of Mouse MFGE8 in the samples by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Mouse MFGE8 in samples. No significant cross-reactivity or interference between Mouse MFGE8 and analogues was observed.
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃/-20℃, 12 months
Shipping: ice bag