Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit
Size: 96T
Catalogue Number: E-BC-K014-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: NEFA
Research Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism
Result Type: Quantitative
Detection Instrument: Microplate reader, Biochemistry analyzer
Tested Sample Types: Serum, plasma, tissue homogenate, cells, cell supernatant
Assay Time: 30 min
Detection Type: Colorimetric method
Detection Range: 0.01-3.0 mmol/L
Test Principle: NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS) . Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD) . Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD) . The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag