p21 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-66357-60, E-AB-66357-120, E-AB-66357-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: p21
Target Synonym: CAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; p21CIP1; CDKN1A
Research Areas: Cell Biology, Epigenetics and Nuclear Signaling, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P38936
Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human p21 (NP_000380.1).
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000 IF 1:50-1:200
Calculated MW: 21kDa
Observed MW: 21kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only