PCNA Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-40489-60, E-AB-40489-120, E-AB-40489-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: PCNA
Target Synonym: ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; PCNA; Pcna/cyclin; PCNA; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P12004
Background: Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.
Concentration: 1mg/mL
Immunogen: Synthetic peptide of Human Proliferating cell nuclear antigen
Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
Purification Method: Antigen Affinity Purification
Dilution: WB 1:3000-1:7000
Calculated MW: 28 kDa
Observed MW: 35 kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only