PDHA1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-60493-60, E-AB-60493-120, E-AB-60493-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: PDHA1
Target Synonym: PDHA1; PDHA; PDHAD; PDHCE1A; PHE1A
Research Areas: Cancer, Metabolism, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P08559
Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human PDHA1 (NP_000275.1).
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Calculated MW: 40kDa/43kDa/44kDa/47kDa
Observed MW: 45kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only